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Single molecule array (Simoa?) Testing Service (超敏感ELISA測試)
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實驗原理
Simoa跟傳統ELISA一樣都是sandwich的方式，差別在於Simoa可以偵測每一個在beads上的抗原抗體複合物，偵測”digital “訊號，只要有一個分子有接上抗原即可被偵測到，因此較傳統ELISA靈敏
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其他數據
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Reference
- Single Molecule Arrays (Simoa) for the Ultra-Sensitive Detection of the Host Immune Response to Dengue Infection?
February 18, 2015?
Shonda T. Gaylord, Sama Abdul-Aziz, David R. Walt
J. Clin. Microbiol., February 18, 2015
doi:10.1128/JCM.03487-14 - Emerging Technologies to Increase Ligand Binding Assay Sensitivity?
January 2015?
Saloumeh K. Fischer et. al.
The AAPS Journal, Vol. 17, No. 1, January 2015
DOI: 10.1208/s12248-014-9682-8 - Anew ultra-high sensitivity troponin I assay for chest pain patients with no evidence of troponin I using a conventional assay?
December 4, 2014?
Wu AHB, van Wijk XMR
Clin Biochem 2014
http://dx.doi.org/10.1016/j.clinbiochem.2014.11.018 - Next Generation Ligand Binding Assays—Review of Emerging Technologies’ Capabilities to Enhance Throughput and Multiplexing?
November 2014?
Johanna Mora et al
The AAPS Journal, Vol. 16, No. 6, November 2014
DOI: 10.1208/s12248-014-9660-1 - High Sensitivity Cardiac Troponin Assays in the Clinical Laboratories?
October 22, 2014?
Petr Jarolim
Clin Chem Lab Med 2014
DOI 10.1515/cclm-2014-0565
- CSF and Plasma Amyloid-b Temporal Profiles and Relationships with Neurological Status and Mortality after Severe Traumatic Brain Injury?
October 10, 2014?
by Stefania Mondello, Andras Burk, Pal Barzo, Jeff Randall, Gail Provuncher, David Hanlon, David Wilson, Firas Kobeissy & Andreas Jeromin - BloodBiomarkers for Brain Injury in Concussed Professional Ice Hockey Players?
March 13, 2014?
JAMA Neurology. Published online March 13, 2014. doi:10.1001/jamaneurol.2014.367?
Pashtun Shahim, MD; Yelverton Tegner, MD, PhD; David H. Wilson, PhD; Jeffrey Randall, PhD3; Tobias Skillb?ck, MD; David Pazooki, MD, PhD; Birgitta Kallberg, BSc; Kaj Blennow, MD, PhD; Henrik Zetterberg, MD, PhD - Tau proteins in serum predict neurological outcome after hypoxic brain injury from cardiac arrest: Results of a pilot study
March 2013?
Resuscitation Journal?
J, Mortberg E, Provuncher G, Fournier D, Duffy D, Rubertsson S, Blennow K, Zetterberg H, Wilson D. - Plasma tau levels in Alzheimer's disease
March 28, 2013?
Alzheimer's Research & Therapy?
Henrik Zetterberg, David Wilson, Ulf Andreasson, Lennart Minthon, Kaj Blennow, Jeffrey Randall and Oskar Hansson
- Olympic boxing is associated with elevated levels of the neuronal protein tau in plasma
March 8, 2013
Brain Injury
Neselius S, Zetterberg H, Blennow K, Randall J, Wilson D, Marcusson J, Brisby H. - Single?molecule enzyme-linked immunosorbent assays: Theoretical considerations
Lei Chang, David M. Rissin, David R. Fournier, Tomasz Piech, Purvish P. Patel, David H. Wilson, David C. Duffy. Journal of Immunological Methods 378 (2012) pp. 102-115 - Single-molecule enzyme-linked immunosorbent assay detects serum proteins at subfemtomolar concentrations.
David M Rissin, Cheuk W Kan, Todd G Campbell, Stuart C Howes, David R Fournier, Linan Song, Tomasz Piech, Purvish P Patel, Lei Chang, Andrew J Rivnak, Evan P Ferrell, Jeffrey D Randall, Gail K Provuncher, David R Walt & David C Duffy
Nature Biotechnology 28, 595–599 (May 2010) doi:10.1038/nbt.164
